NMR spektrometers at 幸运飞艇计划
The Department of Chemistry has three operational high-field NMR-spectrometers, in addition to a 60 MHz benchtop spectrometer.
Main content
The three high-field spectrometers are located in the NNP building and are part of the Norwegian NMR Platform (NNP). Meanwhile, the 60 MHz benchtop instrument is located at Realfagbygget. The spectrometers are:
- 850 MHz: For biomolecules, time consuming experiments, or increashed听resolution.
- 600 MHz: Walk-up instrument for routin liquid-state NMR.
- 500 MHz: Wide-bore spectrometer. Used for solid-state, imaging, and diffusion.
- 60 MHz: Benctop spectrometer.
850 MHz
850 MHz AVANCE III HD spectrometer from Bruker BioSpin. The spectrometer is ideal for the study of large biomolecules, i.e. proteins. In addition, the instrument is suitable for samples that benefit from the higher sensitivity and resolution, or for time consuming experiments.
The instrument is equipped with a TCI CryoProbe (1H, 2H, 13C, and听15N) and an automatic sample changer (SampleJet) with a capasity of 5x96 short tubes (refridgerated, in 5 racks) and 30听positions for long tubes (at room temperature).
The 850 MHz magnet is the core spectrometer of the (Norwegian NMR platform) project, financed by . It was installed in 2016.
600 MHz
600 MHz AVANCE NEO spectrometer from Bruker BioSpin. 听The spectrometer has a versetile role, and is used for everything from organic chemistry to metabolomics and structural biology.
The instrument is equipped with a QCI-P CryoProbe (1H, 2H, 13C, 15N, and 31P) and an automatic sample changer (SampleJet).听Teh SampleJet has five racks for 96 short tubes, where the temperature can be set individual for each rack. Usually, one rack is kept at room temperature, while the others are refridgerated. In addition, it has room for 96 standard-length NMR tubes at听room temperature.
The 600 MHz instrument was financed by , , , and University of Bergen. It was installed 2017.
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500 MHz
500 MHz wide-bore AVANCE III HD spectrometer from Bruker BioSpin. This instrument is tailored for听solid-state NMR,听micro-imaging and diffusion. The following probeheads are available:
- Triple-resonance CP-MAS (Magic Angle Spinning) probehead 1H/13C/15N, and additional听RF inserts (1H/31P/23Na-29Si),听for both 4 mm and 2.5 mm rotors.
- Double resonance modes: 13C/31P; 15N/13C
- Triple resonance modes: 31P/29Si-65Cu; 13C/15N-2H
- 1H channel on the 4mm MAS probe is tunable to 19F. This option will soon be available also for the 2.5mm MAS probe.
- Triple-resonance flat-coil probehead (1H/13C/15N) with a 31P switch (13C-31P switch), for NMR studies听on听oriented samples.
- MICWB40 micro-imaging probehead, with selective 1H RF insert, and double-tuned 1H/X RF insert (on several听diameters).听MICRO2.5 gradient system (2.5 G/cm/A) with amplifiers (X,Y,Z) 2xGREAT -60 (60A).听Using ParaVision for multi-dimensional MRI/MRS data acquisition, reconstruction, analysis, and visualization.
- Variable-temperature Diffusion probehead with Z-gradient: BBI 500W2/S4 H&F-BB-D-05 DIFF.
The instrument was puchased by 幸运飞艇计划 as part of the Norwegian NMR Platform, and it was installed in 2015.
60 MHz
60 MHz Benchtop NMR Spectrometer (NMReady60e) with a compact permanent magnet from Nanalysis Scientific Corporation. The spectrometer is mainly used for teaching, quick walk-up routine experiments, and 19F NMR.
Two RF-channels, 1H and 19F, are available. Various experiments are possible such as T1 and T2 measurements, COSY, JRES and Spin-Echo. Solvent suppression schemes are included (DANTE and Presaturation). Both 1H and 2H field-frequency locking modes are available
The spectrometer is equipped with a sample changer (Nanalysis AUTOsample-60) with a carousel for 25 standard 7鈥欌�� 5 mm NMR tubes.
The 60MHz instrument was financed by the Department of Chemistry and the Centre for Pharmacy at 幸运飞艇计划. It was installed in 2021.
The NMR museum
On the third floor of Realfagbygget there is a small museum exhibit, consisting of two older NMR spectrometers:
- A Bruker DPX 400 MHz spectrometer. It was discharged on May 20th 2015
- A 200-MHz magnet听
In addition, the following spectrometers (not a complete list) have been used at the department:
- Bruker Avance 500 MHz, quenched in 2022.
- Bruker DRX 600 MHz, decomissioned in 2017.
- JEOL JNM-C-60H, the first NMR spectrometer at 幸运飞艇计划, installed in 1967.
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